生物体内NO定量测定方法研究进展

Reactive nitrogen species (RNS) with its diverse functions in biological cells can play an important role in physiology and pathology. Therefore, the establishment of RNS detection method with good accuracy, high sensitivity and convenient operation is essential to provide an important basis for the early diagnosis of certain diseases.This review has summarized different methodologies and techniques such as chemiluminescence, colorimetry, ESR spectroscopy, electrochemical sensors and fluorescence to achieve direct quantitative determination of NO(the most common RNS molecules) and analyzed the mechanism and advantages or disadvantages of each method by contrast. Finally, the outlook has been made to obtain further research and applications of intracellular NO molecular.