活化Notch1信号对人舌鳞癌细胞生长的影响及作用机制的研究

Objective To construct the recombinant retroviruses expressing the constitutively active form of Notch1（intracellular domain of Notch,ICN） and to explore their effects on the growth of human tongue carcinoma cells Tca8113. Methods Notch1 (ICN) genes were inserted into retrovirus expression vector pCLNRX. The expression vector pCLNRX-ICN and packaging vector pCL-10A1 were co-transfected into 293T package cells by lipofectamineTM 2000. The recombinant retroviruses were used to infect human tongue carcinoma cells Tca8113. After infected, proliferation of Tca8113 cells was observed by MTT assay.Notch1 and p21WAF1/CIP1expression was detected by Western blot. Results Recombinant retrovirus vector pCLNRX-ICN was successfully constructed. After infection of recombinant retrovirus, the growth of Tca8113 cells was inhibited significantly. Western blot showed that Notch1 signaling up-regulated p21WAF1/CIP1 expression. Conclusion Constitutively active at exaggerated levels could inhibit the growth of tongue carcinoma cells, at least partially, through induction of p21WAF1/CIP1 expression.