水体Cu2+胁迫对克氏原螯虾（Procambarus clarkii）体液免疫功能的影响

In order to study the effects of copper on the humoral immunity of crayfish Procambarus clarkii，crayfish were exposed to different concentrations of copper at 0, 0.5, 1.0, 3.0, 5.0, 10.0 mg/L. Antibacterial activity (Ua), bacteriolytic activity (Ub), phenol oxidase (PO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were determined after 24 h, 48 h, 72 h and 96 h of exposure, respectively.The results showed that the activities of Ua and Ub were lower than that in the control group (P ≤ 0.05) after 48h and 72h, when crayfish were exposed to the concentrations of 1.0, 3.0, 5.0 and 10.0 mg/L. PO activity reflected the time-dose response relationship, and PO of each treatment group was significantly lower than that of the control after 96h. Activities of SOD and CAT in 3.0, 5.0 and 10.0 mg/L groups was inhibited significantly during entire experimental period, but MDA in each tested group was higher than that of the control after 48h of exposure (P ≤ 0.05). The results suggested that Cu2 + in water had a significant negative effect on the humoral immunity of Procambarus clarkii, which indicated that heave metal pollution had potential threat to the health of crayfish.