中国沙棘HrANR 基因及类黄酮累积与抗旱的关系

nthocyanidin reductase is one of the key enzyme involved in the synthesis of flavonoids. In order to explore the structure of ANR gene, ANR enzyme expression pattern and flavonoid content under drought stress and its correlation, a HrANR gene identified from RNA-seq data of sea buckthorn was screened and analyzed by bioinformatics soft, the expression pattern of HrANR gene in different tissues and the content of flavonoid in leaves was performed. The results were as follows: (1) The ORF of HrANR gene is 1 017 bp, which encodes 338 amino acids. It is a stable hydrophilic protein, and the homologous protein has significant family and genus characteristics. (2) HrANR gene was expressed in roots, stems and leaves of sea buckthorn under drought stress, but the expression trends were different, with an increase followed by a decrease and then an increase in roots, a continuous decrease in stems, and an increase followed by a continuous decrease in leaves. (3) The content of flavonoids in the leaves of sea buckthorn under different levels of stress showed a trend that first increased continuously, then decreased slightly and rose to the highest point after rehydration. The above results indicate that the expression of HrANR gene and the changes in flavonoid content are closely related to the drought resistance of sea buckthorn. The flavonoid content in leaves was positively correlated with drought stress at the beginning of drought stress and negatively correlated with drought stress under severe stress. (4) Leaf expression, stem expression and flavonoid content of HrANR gene were negatively correlated (Pleaf= -0.751 43, Pstem= -0.934) and root expression was positively correlated with flavonoid content (Proot= 0.444). The above results indicate that the expression of HrANR genes and changes of flavonoid content in sea buckthorn are closely related to drought resistance, and it can provide a basis for elucidating the drought resistance mechanism of sea buckthorn.