邻苯二甲酸酯降解菌的分离鉴定及降解特性研究
Isolation and identification of phthalate-degrading bacteria and their characteristics
从人工湿地土壤样品中分离到7株能够以邻苯二甲酸酯（PAEs）为唯一碳源和能源生长的微生物命名为D1-D7。就其综合形态特征，主要生理生化特性和16S rDNA序列分析结果对其进行鉴定，D1，D2与假单胞菌属（Pseudomonas sp.）的同源性分别为100%和98％，D3与肠杆菌属（Enterobacter sp.）的同源性在99％以上，其余4株细菌与红球菌属（Rhodococcus sp.）的同源性都在98%以上。用3天摇瓶间歇试验测定这些菌株对PAEs的降解能力表明, DMP、DBP和DEHP的降解率分别在65%，60%和30%以上，且对PAEs的降解率随侧链烷基链的增长而下降。这7株PAEs降解菌均能在以PAEs的降解中间产物--邻苯二甲酸（PA）为唯一碳源的培养液中，对PA的利用率在18%-39%，这说明它们可能能够通过PA途径来实现PAEs的完全降解。
In this paper, seven bacteria strains were isolated from constructed wetland soil and were named D1-D7 using mixtures of phthalate as the sole source of carbon and energy. The morphological, physiological and biochemical characters of the strains were studied, and through 16S rRNA gene sequence analysis, strains D1and D2 were identified as Pseudomonas sp., strain D3 as Enterobacter sp., the other as Rhodococcus sp. The batch test results showed that the all of the strains could effectively degrade phthalate in three days, the degradation efficiencies of DMP, DBP and DEHP were all above 65%, 60% and 30% respectively. The degradation efficiency decreased with the lengthened alkyl chain of phthalate. The D1-D7 strains all growed in phthalic acid as the sole source of carbon and energy, the utilization of phthalic acid was 18%-39%, which showed that phthalate could be thoroughly degraded by strains D1-D7 through phthalic acid metabolism pathway.
温志丹、李喆、高大文、吴唯民
环境污染、环境污染防治微生物学环境生物学
环境生物学邻苯二甲酸酯16S rRNA生物降解
Environmental biologyPhthalate16S rRNABiodegradation
温志丹,李喆,高大文,吴唯民.邻苯二甲酸酯降解菌的分离鉴定及降解特性研究[EB/OL].(2013-05-09)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201305-123.点此复制
评论