虫草多糖对单侧输尿管梗阻小鼠肾小管上皮间充质转化的影响及机制探讨

Objective: To investigate the effect of cordyceps polysaccharide on the tubular epithelial mesenchymal transition in unilateral ureteral obstructive mice. Methods: CD1 Mice were randomly divided into three groups:control group (group C), unilateral ureteral obstrucion group (group UUO) and UUO treated with cordyceps polysaccharide (group UUO+Cp). Mice subjected to UUO were treated with Cp （160 mg/Kg ）or vehicle respectively 2 days before the operation. Mice were sacrificed at day 7 after UUO operation. The expression of a-smooth muscle actin (a-SMA)、E-cadherin、connective tissue growth factor (CTGF)、integrin linked kinase (ILK) in kidney was assessed by immunohistochemistry and real time PCR. Results: Cp treatment significantly reduced intestitial fibrosis. The expression of a-SMA protein in the treatment group were significantly reduced compared to UUO group. The expression of E-cadherin protein in the treatment group were distinctively higher than those in the UUO group (P<0.01). There was a weak expression of CTGF and ILK in normal kidney, while a marked increasing expression of these markers was found in UUO group (P< 0.01). The expression of CTGF and ILK protein in the treatment group were distinctively inhibited compared to UUO group (P<0.01). Conclusion: Cp could significantly inhibit the over expression of CTGF and ILK in obstructed kidney, suppressing the loss of E-cadherin and the expression of α-SMA, which indicated that Cp could play an inhibitive role in the progression of renal tubulointerstitial fibrosis.