电针对局灶性脑缺血再灌注大鼠迟发性细胞凋亡的影响

Objective: To observe the effects of electroacupuncture(EA) against apoptosis in rat with focal cerebral ischemia/reperfusion (IR) injury and its mechanism. Methods: Sixty male SD rats of clean grade were randomly divided into sham operation group, model group and EA group (n = 20), Model group and EA group rats were modified the middle cerebral artery occlusion(MCAO) models with nylon monofilament, the filament was left in place for 60 min and then withdrawn to let the blood reperfuse. EA group was stimulated with EA (at the acupoints of Baihui, Shuigou, Zusanli, sparse and dense wave alternately, frequence of 2HZ/15HZ) for 30 min, repeated the stimulation every other 12 h. After 72 h of reperfusion, TUNEL staining was performed to observe the effect of EA against neuron apoptosis after IR, Western blotting was performed to observe the effect of EA against the apoptins of Bax and Bcl-2. Results: Neuron apoptosis in brain tissue of the EA group significantly alleviated than the model group. The ratio of Bax/Bcl-2 was significantly reduced than the model group. Conclusion: The stimulation of EA can significantly reduce the number of apoptotic neurons in brain tissue, and the protective effect is realized by adjusting the rate of Bax/Bcl-2.