不同气味对大鼠尿液蛋白质组的影响

o rats have corresponding changes in their urinary proteome when smelling odors? Do sniffing different odors produce different changes? In this study, urine samples were collected from six rats after smelling sesame oil and essential balm for three days, and on the third and fourth days, and the samples were analyzed in groups and single before and after comparisons using LC-MS/MS technology. The identified differential proteins were also compared with those produced by the growth and development of rats of the same age group to exclude the influence of growth and development on the results of this experiment. The experimental results showed that comparing the urine protein groups of Day0 and Day4 of the sesame oil group, 143 differential proteins could be identified after screening, and the average number of randomly generated differential proteins was 7.32, at least 94.88% of the differential proteins were not randomly generated. Upon comparative analysis in groups and singly before and after, the same odor showed more consistent changes, and differential proteins such as low-density lipoprotein receptor-related protein 2 and fetuin B, a biomarker of COPD, which are associated with olfactory production, were identified in the sesame oil group, while uteroglobulin, trichothecene factor 3, and visfatin 2 were identified in the essential balm group, which had significant changes and were related to the production of olfactory sensation. It is noteworthy that we identified odor-binding protein 2A again in the essential balm group, simultaneously present in the differential proteins produced by a single before-and-after comparison in four rats, which is consistent with the results presented in the e-cigarette model. This study demonstrates that odor affects the urinary proteome of rats, with different odors affecting it differently. This provides a new approach to explore the biological process of olfaction.<br />&nbsp;