|国家预印本平台
首页|连作木薯对根际与非根际土壤真菌群落结构演替的影响

连作木薯对根际与非根际土壤真菌群落结构演替的影响

Effects of continuous cropping on fungal community structure succession in rhizosphere and non-rhizosphere soils of cassava

中文摘要英文摘要

为揭示木薯连作障碍形成机制,该研究以定点大田连作木薯三年,利用高通量测序技术和生物信息学手段研究连作年限对木薯根际与非根际土壤真菌群落结构演替的影响。结果表明:(1)连作对木薯根际与非根际土壤真菌群落α多样性及群落的分类和发育系统β多样性影响显著。(2)木薯的主要优势菌门是子囊菌门、SAR 超类群、担子菌门、毛霉门和unclassified_k__Fungi,主要优势菌纲为粪壳菌纲、散囊菌纲、座囊菌纲和Intramacronucleata。非根际土壤中,子囊菌门组成变化较大,从第一年的漆斑菌属、粪壳菌纲、座囊菌纲向单一的Knufia 属演化;根际土壤的真菌则从子囊菌门的肉座菌目、刺盾炱目、漆斑菌属、座囊菌纲、粪壳菌纲向norank_d__Eukaryota 门Monosiga 属演化。(3)土壤pH、有机质含量、碱解氮含量、有效磷含量、速效钾含量、过氧化氢酶活性对土壤真菌群落变化影响显著,尤其影响子囊菌门、SAR 超类群、担子菌门和毛霉门的分布。综上所述,木薯连作会引起根系分泌物累积,改变土壤理化性质及真菌的生存环境,进而引起真菌群落多样性和丰富度的变化;其中,非根际土壤中子囊菌由漆斑菌属、粪壳菌纲、座囊菌纲向单一的Knufia 属演变;根际土壤中子囊菌门的肉座菌目、漆斑菌属、粪壳菌纲等有益真菌的相对丰度随连作年限的增加而降低,进而引发木薯连作障碍。

For revealing the mechanism of cassava continuous cropping obstacle formation, the current studyplanted cassava in a fixed field for three consecutive years, and applied high-throughput sequencing technologyand bioinformatics to explore effects of continuous cropping on fungal community structure of both rhizosphereand non-rhizosphere of cassava soils. The results were as follows: (1) Continuous cropping caused significanteffects on the diversity and diversity of rhizosphere and non-rhizosphere soils of cassava. (2) The dominantfungi phyla of cassava rhizosphere and non-rhizosphere soils were Ascomycota, SAR_k__norank,Basidiomycota, Mucoromycota, and unclassified _k __Fungi, while the main classes were Sordariomycetes,Eurotiomycetes, Dothideomycetes and Intramacronucleata. The composition of Ascomycota of thenon-rhizosphere soils varied with the continuous cropping years significantly. In the rhizosphere, Hypocreales, Chaetothyriales, Myrothecium, Dothideomycetes, and Sordariomycetes were observed in 2019 and 2020, whileMonosiga was found in 2021. (3) Soil pH, organic matter content, available nitrogen content, availablephosphorus content, available potassium content, and urease activity significantly influenced the structure offungal communities, especially for distributions of Ascomycota, SAR_k__norank, Basidiomycota, andMucoromycota. In conclusion, continuous cropping of cassava influenced soil physicochemical propertiesthrough the accumulation of root exudates, thus altering the survival environment of fungi, leading todifferences of the diversity and the abundance of fungal communities between cassava rhizosphere andnon-rhizosphere soils. The relative abundance of Hypocreales, Myrothecium, and Sordariomycetes decreasedwith continuous cropping years, which may be one of main causes of the continuous cropping obstacles ofcassava.

覃锋燕、韦茂贵、阳太亿、甘李、申章佑、黄苑航、彭晓辉、黄艳英、彭晓雪、欧桂宁

DOI:10.11931/guihaia.gxzw202311024

农业科学研究植物保护植物学

木薯,连作,真菌,根际与非根际土壤,微生物多样性

cassava (Manihot esculenta) continuous cropping fungi rhizosphere and non-rhizosphere soils microbial diversity

覃锋燕,韦茂贵,阳太亿,甘李,申章佑,黄苑航,彭晓辉,黄艳英,彭晓雪,欧桂宁.连作木薯对根际与非根际土壤真菌群落结构演替的影响[EB/OL].(2024-05-15)[2025-08-23].https://chinaxiv.org/abs/202405.00168.点此复制

评论