叶下珠提取物抑制急性乙肝小鼠模型中HBV复制及表达的实验研究

Objective To observe the effects of the extract from Phyllanthus urinaria L. on HBV replication and expression in a murine model of acute hepatitis B virus infection. Methods The eukaryotic expression plasmid pHBV1.3, which contains 1.3-fold-overlength genome of HBV, was transfected into C57b/6 mice by hydrodynamic injection via tail vein, to establish a murine model of acute hepatitis B virus infection. The extract from Phyllanthus urinaria L. was prepared in 0.5 g/ ml, 0.25 g/ ml, 0.125 g/ ml , respectively, in sterile PBS. From the 1st day after infection, the mice were treated by intragastric administration with Phyllanthus urinaria L. at different concentration for 3 days. On days 4 after infection, chemiluminescence method was used to detect the concentration of HBs Ag and HBe Ag in mice serum; PCR method was used to detect the level of HBV DNA in serum; |immunohistochemistry was used to detect the expression of HBc Ag and HBs Ag in mice liver. Results The concentration of HBs Ag and HBe Ag in mice sera were decreased obviously by the drug in a dose-dependent manner, compared to the infected mice (P<0.05). Although the drug at all concentration could reduce HBV DNA concentration in mice sera, only that in the highest dose robustly reduced HBV DNA level, compared to the infected mice (P<0.05). And the drug in 0.5 g/ ml concentration obviously inhibited the HBc Ag and HBs Ag expression in mice liver. Conclusion The extract from Phyllanthus urinaria L. obviously inhibited HBV replication and expression in a murine model of acute hepatitis B virus infection and exerted distinctive anti-HBV effects.?????