二甲双胍保护心肌缺血再灌注损伤的分子的机制

im: The objective was to examine the protective effect of metformin (Met) on myocardial ischemia-reperfusion (IR) injury and It's molecular mechanism. Methods: Rat Langendorff IR test was used in this study, Left ventricular (LV) function, infarct size, CK-MB release and TUNEL staining were performed, Meanwhile, western blot was used to examine the expression of antioxidant enzymes and apoptotic proteins in vitro. Results: Met treatment significantly improved LV function, reduced infarct size and CK-MB release in comparison with IR group. Up-regulated expression of MnSOD, as well as down-regulated expression of apoptotic proteins. Decreased TUNEL staining positive cells were also observed in Met group. Conclusion: Met exerted protective effect on myocardial IR injury, and this effect was related to the up-regulation of antioxidant enzymes and down-regulation of apoptotic proteins.