肠上皮特异性缺失Bmal1改变肠道糖脂吸收相关基因的表达

he gut is essential to maintaining glucose homeostasis, lipid metabolism, and absorption. In order to investigate the effects of specific loss of Bmal1 in intestinal epithelium on lipid and glucose absorption in mice, we used intestinal epithelium-specific knockout mice (Bmal1 flox/flox; villin-Cre, Bmal1ΔIEC) model. Under the conditions of Normal fat diet (ND) or High fat diet (HFD), the weight changes of mice were monitored, and the expression of genes related to glucose and lipid absorption was detected by qRT-PCR. The results of this study are as follows: 1) Bmal1ΔIEC mice acquire weight at a slower rate than control mice on a normal diet, and the expression of genes involved in fat absorption in the small intestine epithelium is down-regulated; 2) When given a high-fat diet, Bmal1ΔIEC mice gained weight at a rate similar to that of control mice, and the expression of genes involved in cholesterol absorption was up-regulated in the small intestine epithelium while that of genes involved in triglyceride biosynthesis was down-regulated; 3) Whether given a normal diet or a high-fat diet, the expression of genes involved in glucose absorption in the small intestine of Bmal1ΔIEC mice In this study, it was discovered that a specific loss of the biological clock gene Bmal1 in the intestinal epithelium resulted in weight changes in mice and altered the expression of genes related to intestinal epithelial lipids and glucose absorption. This finding suggests that the biological clock gene Bmal1 was crucial in controlling glucose absorption and weight changes.