β-防御素抑制巨噬细胞自噬促进铜绿假单胞菌清除

Objective: To investigate the role and mechanism of β-defensins in macrophage-mediated immune defence of Pseudomonas areuginosa (PA). Methods: THP-1 macrophages were treated with BD2, BD3 or rapamycin, followed by PA infection. Flow cytometry and bacterial killing assay were used to detect macrophage-mediated phagocytosis and intracellular killing of PA. Protein levels of LC3 were detected by Western Blot and LC3 puncta were observed by immunefluorescence microscopy. Results: β-defensins inhibited macrophages autophagy and autophagy inhibited phagocytosis and bacterial killing of PA. β-defensins inhibited autophagy to promoted macrophages--mediated phagocytosis and intracellular killing of PA. Conclusions: β-defensins inhibited autophagy to promoted macrophages--mediated clearance of PA.