长春花碱和秋水酰胺诱导TK6和TK6-E6细胞TK基因突变比较研究
omparison of TK gene mutation assay in TK6 and TK6-E6 cell induced by Vinblastin and Colcemid
目的 比较TK6和TK6-E6人类淋巴母细胞TK基因突变试验对两种纺锤体毒物－长春花碱(vinblastin VBL)和秋水酰胺(Colcemid CCM)检出情况的差异。方法 用长春花碱和秋水酰胺处理TK6和TK6-E6细胞24小时，进行常规TK基因突变试验。结果 长春花碱和秋水酰胺对TK6和TK6-E6细胞均有致突变性，TK6细胞的诱发和自发突变频率低于TK6-E6,而TK6-E6细胞的小集落百分比高于TK6细胞（P<0.05）。结论 两种细胞都可用于TK基因突变试验，但由于TK6-E6所用细胞量少，倍增时间较短，故建议使用TK6－E6细胞。
Objective To compare TK gene mutation assay in human lymphoblastoid cell lines TK6 and TK6-E6 induced by vinblastin and colcemid. Methods Regular TK gene mutation assays were experimented after TK6 and TK6-E6 human lymphoblastoid cells were treated with vinblastin and colcemid for 24h.Results TK6 and TK6-E6 cells both were induced by vinblastin and colcemid.The TK6’s nature mutation frequency and induced mutation frequency were lower and the TK6-E6’s SC% were higher. Conclusion Both TK6 and TK6-E6 cell lines can be used in TK gene mutation assay , but TK6-E6 cell line was recommended to be used in the assay for its less quantity and doubling time.
张立实、丁晓琴、王亚男、帅培强
基础医学遗传学细胞生物学
K基因突变试验 TK6 TK6-E6 长春花碱 秋水酰胺
K mutation assayK6K6-E6Vinblastinolcemid
张立实,丁晓琴,王亚男,帅培强.长春花碱和秋水酰胺诱导TK6和TK6-E6细胞TK基因突变比较研究[EB/OL].(2005-12-14)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200512-360.点此复制
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