超高效液相色谱-串联质谱测定蔬菜鲜样中喹诺酮类抗生素

n analytical method for determining four quinolone antibiotics in fresh vegetable using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed. Five gram of ground fresh vegetable sample was extracted using 10 mL of acetic acid-acetonitrile (1/100,V/V) under vortex- ultrasonic treatment.The extract was purified and concentrated by HLB cartridge, followed by eluting the HLB cartridge using 4 mL of acetic acid-acetonitrile (1/100,V/V). Using 0.1% formic acid in acetonitrile and 0.1% formic acid as the mobile phase, the analyte was determined with UPLC-MS/MS under gradient elution approach. The recoveries of four quinolone compounds in fresh vegetable samples were from 83% to 102%, with relative standard deviation (RSD) less than 10%. Limit of detection and limit of quantification ranged from 0.003 to 0.199 μg/kg and from 0.009 to 0.662 μg/kg, respectively. The analytical method was successfully used to determine four quinolone antibiotics in fresh vegetables from supermarkets of Guangzhou city, which suggested a health concern related to pollution of quinolone antibiotics in vegetable.