不同类群动物胰蛋白酶结构特征与酶学性质关系

rypsin (EC 3.4.21.4) is one of the major proteinases in animal digestive system and acts as a key mediator during the food assimilation and zymogen activition of animals, and exuviation and immunity improving of insects and crustuceans. 112 sequences obtained from Genebank were divided into five groups, and relationship between the structure features and enzymologic properties of trypsins from insects, temperate and tropical zone crustacean, cold adapted fish, temperate and tropical zone fish and higher vertebrate are conducted. It was indicated that, the evolutionary trend between the nummbers of amino acids, molecular diameter, diversity of molecular structure and fuction of arthropods and vertebrates trypsins are different, as two groups with highest evolutionary position within invertebrates and chordates separately. The animal trypsin active centre and amino acid side chains round are strictly conservative. With different Arg, Glu, Asp, His, Cys, Pro, Tyr and Met around periphery and active centre, significant difference of stability, flexibility and peaction energy consumption are present. The Kcat of CAF-TRY, TTF-TRY and TTC-TRY are increased, or the Km of which are reduced, hence the CAF-TRY catalytic efficiency > TTF-TRY≥TTC-TRY ≥I-TRY and HV-TRY.