小鼠CLOCK基因对食欲素orexin的调节作用

lock gene plays a central role in circadian system. In recent years, the study found that clock gene also has relationship with obesity and sleep duration. It seems plays the same role with orexin whose regulation function in obesity and sleep has been proved before. In previous research, we find that orexin which express in hypothalamus has its own circadian rhythm in mice. In order to explore whether exists regulation between clock and orexin, we inject clock siRNA to mice paracele, measuring the mRNA and protein expression level of orexin with Real-time PCR and western-blot, finding that there is no statistical differences between experimental group and controlled group. To further determine the clock role in regulating function of orexin, we use immunofluorescent double staining with orexin and c-fos, the result shows that orexin positive neurons express activity decreased significantly when clock been influenced successfully.