G1和双酚A对血管内皮细胞窖蛋白-1表达及蛋白相互作用的影响
he effects of G1 and BPA on cavelin-1 in vascular endothelial cells and the interaction between the proteins-related
目的 探究G1（GPER 特异性激活剂）和双酚A（BPA）对血管内皮细胞窖蛋白-1（Cav-1）以及Cav-1和G蛋白偶联雌激素受体（GPER）相互作用的影响。方法 不同剂量的双酚A或G1干预下，Western Blot方法检测内皮细胞中Cav-1、HSP90蛋白表达水平和PKA磷酸化水平和免疫荧光检测Cav-1和GPER之间的相互作用。结果 高浓度的双酚A或G1都能上调内皮细胞Cav-1蛋白的表达；其中G1可促进Cav-1和GPER的相互作用，双酚A可降低HSP90蛋白表达水平并上调PKA信号蛋白的磷酸化水平。结论 1. GPER作用的发挥与Cav-1密切有关；2. 双酚A影响血管内皮细胞Cav-1的表达调控以及HSP90的表达水平，并可能通过PKA信号通路促进内皮的增殖和迁移。
To explore the effects of G1 and bisphenol A on cavelin-1 in vascular endothelial cells and the interaction between Cavelin-1 (Cav-1) and G protein-coupled estrogen receptor (GPER). Methods: After different doses of BPA or G1 for 24 h treatment, expression levels of Cav-1, HSP90, and PKA phosphorylate substrate was measured by Western Blot. The interaction between Cav-1 and GPER under the treatment of G1 was detected by immunofluorescence. Result: High concentration of bisphenol A and G1 up-regulated the expression of Cav-1. G1 stimulated the interaction between the GPER and Cav-1, while the phosphorylation levels of PKA and the expression of HSP90 were elevated by bisphenol A. Conclusion: 1.The function of GPERB exhibits close connection to Cav-1. 2. Bisphenol A, which possibly results in proliferation as well as migration in endothelial cells through downstream proteins PKA on Cardiovascular System, also affects the expression of caveolae and HSP90.
丁世斌、许芳溢、金鑫、应晨江、张丕伟、田翀
基础医学生物化学生理学
G蛋白偶联受体双酚AG1窖蛋白-1蛋白激酶A
GPERbisphenol AG1Cav-1PKA
丁世斌,许芳溢,金鑫,应晨江,张丕伟,田翀.G1和双酚A对血管内皮细胞窖蛋白-1表达及蛋白相互作用的影响[EB/OL].(2014-12-30)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201412-937.点此复制
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