大鼠海马突触质膜蛋白质复合物研究

Synapses are specialized sites of information exchange between neurons. Many diseases, such as drug addition and mood disorders, are likely results from the altered expression of synaptic protein involved in neuro-transmission or neuroplasticity. A detailed description of native protein complexes in synaptic plasma membrane is therefore essential for understanding the related mechanisms and disease processes. In this study, we applied two-dimensional Blue-SDS Gel Electrophoresis in combined with the state-of art mass spectrometry to study the protein complexes in SPM from rat hippocampus. A prefractionation strategy by adjusting the pH of TritonX-100 extraction of isolated SPM was used in the sample preparation . As a result, more than 500 non-redundant proteins were indentified, of which 31.5% were integral membrane proteins and 13.0% were lipid-anchored proteins. Almost no nucleus and ribosomal proteins were detected according to the GO annotations. A lot of knowned and unknowned protein complexes were found. Our findings here should shed light on understanding the physiology and pathology of synapse.