内滤效应介导的可逆型氧化还原生物发光探针

he balance of the redox state in the organism is closely related to the normal physiological activities of the body and the stable operation of the physiological metabolic process in the aerobic environment. If there is a persistent abnormality in the concentration of redox-active species in the organism, it will lead to the imbalance of redox balance, which will induce various diseases. Compared with traditional photoluminescence imaging technology, bioluminescence imaging does not require external excitation to generate optical signals, and exhibits the advantages of high sensitivity and high signal-to-noise ratio in biological imaging. However, the current bioluminescent probes based on the substrate cage strategy have irreversible defects. In order to solve this problem, this paper uses the principle of inner filter effect to convert the absorption signal change into the bioluminescence signal change, and constructs the H2O2/SO2 redox pair cycling response on the Huaqing-like absorber that overlaps with the bioluminescence spectrum of the luciferin substrate. It can regulate the bioluminescence signal generated by the luciferin substrate-enzyme reaction instead of the bioluminescence reaction itself, so as to realize the reversible cycle detection of redox active substances, and apply it to the dynamic imaging analysis of intracellular H2O2/SO2 redox .