茉莉酸甲酯调控阳春砂HMGR、DXR和DXS基因表达

Objective To investigate the rule of the MeJA regulating the expression of HMGR, DXR and DXS, which are the genes encoding the key enzymes of terpenoids biosynthetic pathway in Amomum villosum Lour. Methods The seedlings of A. villosum were sprayed with 20 μmol/L and 200 μmol/L MeJA. RNA was extracted from the leaves collected at different time after MeJA inducing. The expression levels of AvHMGR, AvDXR and AvDXS genes were detected by Real-time quantitative PCR. Results The expression of AvHMGR, AvDXR and AvDXS could be up-regulated by the induction of MeJA. The stimulation of AvDXR was most significant and that of AvHMGR was less significant. The highest expression levels of AvDXR, AvHMGR were 9.40 and 3.95 times as high as the untreated sample respectively. AvDXR was induced significantly by both high and low concentration MeJA, however, AvHMGR was only sensitive to the induction of MeJA with low concentration and AvDXS was insensitive to the induction of either high or low concentration MeJA. The expression of AvHMGR and AvDXR reached the highest level at 24 h and then returned to the former level at 72 h after the induction. Conclusion The regulation of elicitor MeJA inducing AvDXR and AvHMGR expression is excitation formula. The expressional levels of the genes reached the highest level rapidly and then fell back to the former level. This study provided the theory basis for using MeJA and AvHMGR, AvDXR to process the regulation of terpenoid metablic engineering for A. villosum.