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还原性硫化物浓度对排硫硫杆菌固碳能力的影响及其作用机制

he effect of reducing sulfide on the carbon fixation capability of Thiobacillus thioparus and its mechanism

中文摘要英文摘要

以Na2S2O3为代表,研究了还原性硫化物浓度对排硫硫杆菌固碳能力的影响,并结合不同浓度Na2S2O3条件下的硫化量和固碳关键酶RubisCO编码基因cbb的转录特性,阐明还原性硫化物影响固碳能力的作用机制。结果表明,增加Na2S2O3的浓度能显著提高排硫硫杆菌的固碳能力和硫化能力,另外基因及其转录水平分析结果表明,Na2S2O3对cbb基因的转录水平没有直接的影响,且对RubisCO酶的表达模式基本无影响。因此推测Na2S2O3影响排硫硫杆菌固碳能力的作用机制可能是作为电子供体被氧化以提供能量,进而促进细胞生长,增强固碳能力,而并不改变cbb基因的转录特性。

he effect of reducing sulfide on the carbon fixation capability of Thiobacillus thioparus (DSM 505) was determined by using Na2S2O3 as the reducing sulfide. In addition, the mechanism was clarified by analyzing the sulfide-oxidizing amount and the transcription characteristics of RubisCO- encoding genes (cbb genes). The result showed that by increasing the concentration of Na2S2O3, the carbon fixation capability and the sulfide-oxidizing amount of Thiobacillus thioparus can be promoted. Moreover, the analysis from the levels of genes revealed that Na2S2O3 has no significant effect on the transcription level and transcription pattern of cbb genes. So Na2S2O3 might improve the carbon fixation capability by being oxidized as electron donors to provide the cells with energy.

王磊、王亚楠、李欢

环境生物学微生物学生物化学

O2同化排硫硫杆菌硫氧化电子供体核酮糖-1 5-二磷酸羧化酶/加氧酶

O2 assimilationThiobacillus thioparussulfide oxidationelectron donorsRubisCO

王磊,王亚楠,李欢.还原性硫化物浓度对排硫硫杆菌固碳能力的影响及其作用机制[EB/OL].(2016-06-01)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201606-87.点此复制

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