大豆分离蛋白-g-PAMPS接枝物的合成与性能研究
Synthesis and Aqueous Solution Properties of Graft Copolymers Based on Soy Protein Isolate with Amino-terminal PAMPS
用巯基乙胺（AET）为链转移剂，过硫酸铵（APS）为引发剂合成了氨基封端的聚2-丙烯酰胺基-2-甲基丙磺酸（H2N-PAMPS）。再用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺（NHS）在室温下催化H2N-PAMPS的端氨基与大豆分离蛋白（SPI）的羧基反应形成酰胺键，得到接枝物SPI-g-NH-PAMPS。用1H-NMR、傅利叶红外光谱、13C-NMR对接枝物H2N-PAMPS和SPI-g-NH-PAMPS进行结构表征。用Zeta电位仪、紫外可见光光谱，荧光光谱，动态激光光散射对SPI及其接枝物SPI-g-NH-PAMPS的水溶液性质进行了研究。结果表明：与SPI相比，SPI-g-NH-PAMPS的溶解性、表面疏水性、乳化性等有所改善，接枝物SPI-g-NH-PAMPS聚集体粒径增大，形成独特的核壳结构。EDC催化接枝反应为大豆分离蛋白改性提供了一种新途径，将拓宽大豆分离蛋白的应用。
he graft copolymers (SPI-g-NH-PAMPS) were prepared by a graft reaction between the free carboxylic acid groups of soy protein isolate (SPI) and the amino groups of poly(2-Acrylanmido-2-methyl propane sulfonic acid) (H2N-PAMPS) using 1-(3-(dimethylamino)propyl)-3-ethyl-carbodiimide hydrochloride (EDC)/ N-Hydroxysuccinimide (NHS) as the condensing system in a buffer solution. H2N-PAMPS and SPI-g-NH-PAMPS were fully characterized by 1H-NMR, FTIR and 13C-NMR spectra. The aqueous solution properties of SPI and SPI-g-NH-PAMPS were studied by Zeta potentiometer, UV-Visible spectroscopy, fluorescence spectrometer, dynamic laser light scattering (DLS). The results showed that: compared with the SPI aqueous solution, the solubility, surface hydrophobicity, emulsifying properties and surface charges of SPI-g-NH-PAMPS aqueous solution have been obviously improved. SPI-g-NH-PAMPS aggregates present a unique core-shell structure, and the average size of SPI-g-NH-PAMPS aggregates is bigger than that of SPI aggregates. The graft reaction using EDC provides a new method to modify SPI and expands the application field of SPI.
姜鹏、陈明清、周华、江金强、刘晓亚、白绘宇、杨成
生物化学生物工程学高分子化合物工业
大豆分离蛋白EDC2-丙烯酰胺基-2-甲基丙磺酸（AMPS）水溶液性质EM
Soy protein isolateEDC 2-Acrylanmido-2-methyl propane sulfonic acid Aqueous solution properties TEM
姜鹏,陈明清,周华,江金强,刘晓亚,白绘宇,杨成.大豆分离蛋白-g-PAMPS接枝物的合成与性能研究[EB/OL].(2008-06-17)[2025-06-10].http://www.paper.edu.cn/releasepaper/content/200806-404.点此复制
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