PTSD大鼠杏仁核神经元凋亡及TMP酶变化的研究

Objective Study on apoptosis and Thiamine Monophosphatase (TMP) expression of amygdala neurons in rats of PTSD. Methods A total of 50 male Wistar rats were randomly divided into 1 d, 4 d, 7 d, 14 d groups of SPS and normal control group. Apply Annexin V-FITC/ PI double-labeled flow cytometry to analysis PTSD rats neurons in amygdala; Using transmission electron microscopy observation of neurons in the amygdala ultrastructural changes; Using enzymes to detect neurons in amygdala activity of TMP expression. Results Amygdala neuronal apoptosis was significantly increased after SPS, TMP activity was significantly enhanced more than the normal control group ,both of reached the peak at 4d and graddually decreased at 7d and 14d. Conclusion Apoptosis happened in amygdala neurons of PTSD rats, the apoptosis ratio increasing is associated with the enhancement of TMP activity in SPS model. This suggest that the TMP is involved in neuronal apoptosis products phagocytosis in amygdala.