Multidimensional Chromatin Regulation of Cell Lineage Differentiation in a Metazoan Embryo
Multidimensional Chromatin Regulation of Cell Lineage Differentiation in a Metazoan Embryo
SUMMARY How chromatin dictates cell differentiation is an intriguing question in developmental biology. Here, a reporter gene integrated throughout the genome was used as a sensor to map the chromatin activity landscape in lineage-resolved cells during C. elegans embryogenesis. Single-cell analysis of chromatin dynamics across critical dimensions of cell differentiation was performed, including lineage, tissue, and symmetry. During lineage progression, chromatin gradually diversifies in general and exhibits switch-like changes following specific cell division, which is predictive of anterior-posterior fate asymmetry. Upon tissue differentiation, chromatin of cells from distinct lineages converge to tissue-specific states but retain “memory” of each cell’s lineage history, which contributes to intra-tissue heterogeneity. However, cells with a morphologically left-right symmetric organization utilize a predetermination chromatin strategy to program analogous regulatory states in early progenitor cells. Additionally, chromatin co-regulation drives the functional coordination of the genome. Collectively, this work reveals the role of multidimensional chromatin regulation in cell differentiation.
Du Zhuo、Wang Yangyang、Zhao Zhiguang、Fan Rong、Xu Weina、Ma Xuehua
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences||University of Chinese Academy of SciencesState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences||University of Chinese Academy of SciencesState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences||University of Chinese Academy of SciencesState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences||University of Chinese Academy of SciencesState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
细胞生物学遗传学分子生物学
Chromatin statePosition-effectsC. elegansSingle-cell analysisEmbryogenesisCell lineageAnterior-posterior asymmetryCell fate specificationLeft-right symmetryCell heterogeneity
Du Zhuo,Wang Yangyang,Zhao Zhiguang,Fan Rong,Xu Weina,Ma Xuehua.Multidimensional Chromatin Regulation of Cell Lineage Differentiation in a Metazoan Embryo[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2020.05.18.101584.点此复制
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