小柴胡汤及其药群配伍抗小鼠H22肝肿瘤的研究

Objective To observe Xiaochaihu Decoction and different herbal formulation of component in inhibiting H22 mice liver cancer and effecting immunological organ, for confirming the inhibiting liver cancer effect of Xiaochaihu Decoction and approaching dose-effect relationship and compatibility mechanism. Method Transplanted tumor H22 liver cancer to mice, filling on high, moderate, low dose of Xiaochaihu Decoction（27g, 13.5g, 6.75g drug/kg）, 5-FU intraperitoneal injection (20mg/kg) as positive control. After being administered for 10 days, we determined cancer weight, body weight, spleen index and thymus gland index for studying dose-effect relationship. And we studied compatibility rule of Xiaochaihu Decoction and the different herbal formulations of its components by using the same method. Result Growth of H22 mice liver cancer was markedly inhibited by high, moderate, low dose group, the inhibiting rates were by 49.66％, 48.52％, 36.91% , optimal administration dose is moderate dose by analyzing. In the study of compatibility rule, only inhibiting rates of C group and A+B+C group exceed 30％, the inhibiting rates were by 41.55% and 43.65％. To compare with normal group, spleen index of three dose groups and the different herbal formulation groups had evidently increased（P<0.01）, thymus gland index of the different herbal formulation groups had significant decreased (P<0.05) , thymus gland index and body weight of 5-FU group had evidently decreased(P<0.001). Conclusion Growth of H22 mice liver cancer was markedly inhibited by Xiaochaihu Decoction and ShenZaoCao group. Inhibiting effect was probably related with elevating tumor-bearing mice body immune function.