亚麻耐盐碱ISSR标记的引物筛选及反应体系的建立
SCREENING ISSR PRIMERS AND ESTABLISHING REACTION SYSTEM IN ALKALI AND SALT TOLERANCE ON FLAX
本研究对亚麻ISSR反应条件进行了优化，并利用100个随机引物，以亚麻耐盐碱材料7000ha-1 和不耐盐碱材料05-10为模板进行ISSR标记筛选。经过三轮的筛选，结果只有33条引物能在亲本间扩增出稳定的多态性，占整个扩增引物的33%，为下一步ISSR标记应用于作图群体分析奠定基础。
he ISSR reaction condition are optimized in this study, ISSR marker screening between the material of alkali and salt tolerance (7000 ha-1) and the opposite material (05-10) for template in 100 random primers. After three rounds of selection, only 33 primers show a stable polymorphism between parents, accounting for 33% of the whole primers amplification, it lay the foundation for the next step in mapping application mark ISSR group analysis.
程莉莉、于莹、姜卫东、康庆华、黄文功、赵东升、吴建忠、关凤芝、刘岩、宋喜霞、吴广文
农业科学研究植物学
亚麻耐盐碱ISSR标记
FlaxAlkali and salt toleranceISSR mark
程莉莉,于莹,姜卫东,康庆华,黄文功,赵东升,吴建忠,关凤芝,刘岩,宋喜霞,吴广文.亚麻耐盐碱ISSR标记的引物筛选及反应体系的建立[EB/OL].(2012-01-04)[2025-05-14].http://www.paper.edu.cn/releasepaper/content/201201-26.点此复制
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