陆地棉茎尖iTRAQ蛋白质组学分析表明植物激素相关信号传导与矮化相关

Identifying important regulative elements and pathways related to dwarf in cotton is a major challenge in cotton breeding. An iTRAQ-based proteomics of stem terminal buds from the upland cotton species Gossypium hirsutum dwarf line LA-1 and high near-isogenic line （NIL） LH-1 was performed. Moreover, the transcriptional expression of differentially expressed proteins (DEPs) belonged to phytohormone signal transduction, hormone biosynthesis and ubiquitin system, were analyzed using the quantitative real-time polymerase chain reaction (PCR). A total of 4, 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of DEPs function as catalytic activity, binding and transporter activity, and involved in metabolism and protein processing related pathways. In particular, 7 DEPs, including 2 of gibberellins (GA) receptor, 3 of cytokinin (CK) receptor, CK oxidase and CK-N-glucosyltransferase were up-regulated in LA-1, and gibberellin 20-oxidase was down-regulated in LA-1. Our results suggest that DELLA-independent GA signaling pathway is primary reason caused the dwarf in LA-1, and indicate that CRE1-2, GID, CKX and GA20ox are potential indicators of dwarf cotton, and the profiling of DEPs may offer a valuable resource for molecular markers of dwarf cotton breeding.