Lecithin:Retinol Acyl Transferase (LRAT) induces the formation of lipid droplets
Lecithin:Retinol Acyl Transferase (LRAT) induces the formation of lipid droplets
Abstract Lipid droplets are unique and nearly ubiquitous organelles that store neutral lipids in a hydrophobic core, surrounded by a monolayer of phospholipids. The primary neutral lipids are triacylglycerols and steryl esters. It is not known whether other classes of neutral lipids can form lipid droplets by themselves. Here we show that production of retinyl esters by lecithin:retinol acyl transferase (LRAT) in yeast cells, incapable of producing triacylglycerols and steryl esters, causes the formation of lipid droplets. By electron microscopy, these lipid droplets are morphologically indistinguishable from those in wild-type cells. In silico and in vitro experiments confirmed the propensity of retinyl esters to segregate from membranes and to form lipid droplets. The hydrophobic N-terminus of LRAT displays preferential interactions with retinyl esters in membranes and promotes the formation of large retinyl ester-containing lipid droplets in mammalian cells. Our combined data indicate that the molecular design of LRAT is optimally suited to allow the formation of characteristic large lipid droplets in retinyl ester-storing cells.
Thiam Abdou Rachid、Houweling Martin、Vaandrager A. Bas、Prinz William A.、Helms J. Bernd、Mari Muriel C.、Caillon Lucie、Chorlay Aymeric、Yadav Kamlesh K.、Toulmay Alexandre、Molenaar Martijn R.、Reggiori Fulvio、Haaker Maya W.、Wubbolts Richard W.、Wassenaar Tsjerk A.
Laboratoire de Physique Statistique, Ecole Normale Sup¨|rieure, PSL Research University, Sorbonne Universit¨|, UPMC Universit¨| Paris 06, Universit¨| Paris Diderot, CNRSDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityNational Institute of Diabetes and Digestive and Kidney DiseasesDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityDepartment of Cell Biology, University of Groningen, University Medical Center GroningenLaboratoire de Physique Statistique, Ecole Normale Sup¨|rieure, PSL Research University, Sorbonne Universit¨|, UPMC Universit¨| Paris 06, Universit¨| Paris Diderot, CNRSLaboratoire de Physique Statistique, Ecole Normale Sup¨|rieure, PSL Research University, Sorbonne Universit¨|, UPMC Universit¨| Paris 06, Universit¨| Paris Diderot, CNRSNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institute of Diabetes and Digestive and Kidney DiseasesDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityDepartment of Cell Biology, University of Groningen, University Medical Center GroningenDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityGroningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen
生物化学分子生物学细胞生物学
LRATvitamin Aretinolretinyl esterretinoidslipid dropletslipid droplet sizenucleationlipid:protein interactionhepatic stellate cellsliver
Thiam Abdou Rachid,Houweling Martin,Vaandrager A. Bas,Prinz William A.,Helms J. Bernd,Mari Muriel C.,Caillon Lucie,Chorlay Aymeric,Yadav Kamlesh K.,Toulmay Alexandre,Molenaar Martijn R.,Reggiori Fulvio,Haaker Maya W.,Wubbolts Richard W.,Wassenaar Tsjerk A..Lecithin:Retinol Acyl Transferase (LRAT) induces the formation of lipid droplets[EB/OL].(2025-03-28)[2025-04-28].https://www.biorxiv.org/content/10.1101/733931.点此复制
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