Purα蛋白质调控p53蛋白乙酰化的研究

Protein modifications are including methylation, acethlation, phosphorylation, ubiquitination and so on. Most of these modifications can change the status of chromosomes, affect the binding of transcription factor with DNA sequence, and regulate gene expression by several mechanisms. Purα is a multifunctional and ubiquitous nucleic acid-binding protein that can bind to both DNA. Purα takes part in the transport and translation of mRNA and the damage repair of DNA by starting DNA replication and transcription regulation.In vivo, p53 exerts a pivotal role in controlling the apoptosis, cell cycle and DNA repair in response to various forms of genotoxic stress. We found that Purα and p53 both carry with the DNA Damage. We constructed pIRES-Flag-Purα vector, and got the stable 293T cell lines of expression Purα.Search the impact of Purα to p53, we found over express Purα that suppress acetylation of p53 at lysine 373.We found that Purα from cytoplasm into nuclear phenomena under the DNA damage conditions through immunofluorescence. The above works have provided a foundation for further research mechanism of Purα in repair of DNA damage.