HIF羟脯化酶在大鼠卵巢细胞中在表达及其定位

im: Using the model of ovarian follicle development of adult female SD rats, we investigated the expression and localization of three isozymes of HIF prolyl hydroylases (PHD1, 2and, 3) in ovarian cells. Methods: 3-4 months old SD female rats were selected to identify their estrous cycle by vaginal smears, and to observe the expression and localization of PHD proteins in ovarian follicular development cycle by two-step immunohistochemistry (IHC) PowerVisionTM (PV). Results: PHD1, PHD2 and PHD3 are all significantly expressed in oocyte, granulosa cells, corpus luteum cells and stromal cells; but the expression of PHD2 significantly more than the amount of PHD1 and PHD3; and their expression changed in stage-specific manner during the follicular developmental cycle in the ovary. Conclusion: PHDs may be directly involved in the regulation of ovarian physiological functions such as ovarian follicles development.