双酚A对雄性斑马鱼的内分泌干扰效应
Endocrine disrupting effect of bisphenol A on Male zebrafish (Danio rerio)
摘要
为了探讨双酚A（bisphenol A, BPA）内分泌干扰效应，本文研究了BPA对斑马鱼肝脏雌激素受体（Esr1）、芳基烃水解酶受体（AhR2）以及卵黄蛋白原（VTG1 mRNA）表达的影响。在96h亚致死浓度范围内设置一个浓度组（2 mgoL-1）对雄性斑马鱼进行曝毒实验。在不同时间点提取肝脏组织总RNA进行定量PCR反应。结果表明BPA暴露12h后, Esr1和VTG1显著提高（P<0.05）。BPA暴露24小时后，Esr1和VTG1mRNA相对表达量依旧保持相当高的水平（P<0.05）。暴露48h后，Esr1的相对表达量继续升高。暴露72h，Esr1和VTG1mRNA相对表达量低于暴露12,24和48h。在BPA暴露的96小时中,暴露48h后，AhR2 mRNA相对表达量为0h的3.22倍。暴露过程中，Esr1和VTG1mRNA相对表达量变化一致。BPA对雄性斑马鱼的Esr1和VTG1mRNA诱导能力较强。但对AhR2诱导能力较弱。结果表明BPA对斑马鱼的内分泌干扰作用存在多途径过程。Abstract
In order to better understand the BPA endocrine disrupting influence on zebrafish and its possible mechanism, the expression of Esr1 (ERα), AhR2 and VTG mRNA in the male zebrafish livers were detected following the exposure to bisphenol A (BPA). The male zebrafish liver RNA was analyzed with the quantity PCR. The results showed that BPA had endocrine disrupting effect on male zebrafish liver: BPA increased the expression of Esr1 and VTG1mRNA in male zebrafish liver, also increased the expression of AhR2 a little. After 12 h treated with BPA，the Esr1 and VTG1 mRNA level significantly increased (P<0.05) and still remained high level at 24h. Esr1 and VTG1 mRNA level was significantly different between the treated groups and the control group (P<0.05). Esr1 mRNA level continued to rise at 48h. After treated with BPA for 72 h，Esr1 and VTG1 mRNA level was lower than at 12, 24 and 48h exposure. During the experiment of 96h exposure, AhR2 mRNA level rose at 48h. Esr1 and VTG1 mRNA changing showed the similar way during the treatments. The results indicated that the BPA's endocrine disrupting in zebrafish could be through a number of ways. 关键词
斑马鱼/双酚A/Esr1(ERα)/AhR2/VTGKey words
zebra fish (Danio rerio)/bisphenol A/Esr1(ERα)/AhR2/VTG引用本文复制引用
曹娜,陶贤继,陈阿琴,魏华,任周.双酚A对雄性斑马鱼的内分泌干扰效应[EB/OL].(2011-12-01)[2025-12-13].http://www.paper.edu.cn/releasepaper/content/201112-26.学科分类
环境生物学/生物科学现状、生物科学发展/环境污染、环境污染防治
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