不同频率电针对抑郁症模型大鼠行为学和β-内啡肽的差异性研究

Objective]To observe the behavioral performance and beta-endorphin levels of depression model rats treated with different frequencies of electro-acupuncture.[Methods] we made the chronic stress-induced depression model rats, which were treated with fluoxetine, sham acupuncture, 2HZ EA and 100HZ EA for seven days respectively; and measured the behavioral performance and beta-endorphin levels of rats. [Results] 2Hz EA group and 100Hz EA group both increased in sugar consumption, horizontal movement and vertical movement comparing with the model group, sham acupuncture group, the fluoxetine group respectively (P<0.05), while there was no difference among fluoxetine group, model group and sham acupuncture group. 2HZ EA group corresponds to 100HZ EA group in sugar consumption and horizontal movement, but 2HZ EA had an advantage in vertical motion (P<0.05).β-endorphin (beta-EP) decreased in 2Hz EA group and 100Hz EA group (P<0.05), and significantly in 2Hz EA group (P <0.05). 2Hz EA and 100Hz EA were both related to beta-EP level, sugar consumption and horizontal movement respectively, but 2Hz EA group shows in the striatum, and 100Hz EA group in the raphe nuclei. [Conclusion] 2Hz EA and 100Hz EA both show antidepressant rapid onset characteristics on depression model of rats, and 2Hz EA is more effective than 100Hz EA. This is related to the decreasing level of beta-EP. The correspondence between beta-EP and behavior performance showed in different parts of the brain caused by the double electro-acupuncture. μ-receptor, the receptor of beta-EP, and its signaling pathway is a further objective of the study.