基于COSM的微通道非侵入式三维温度场测量

he temperature measurements of microfluidics is of great significance in understanding the micro-flow and heat transfer characteristics. The technology based on Computational Optical Sectioning Microscopy (COSM) method combine with Laser-induced Fluorescence (LIF) thermometry is put forward to measure the 3D temperature field in micro-channel. The 3D fluorescence irradiance of dyes mixture solution (Rhodamine B (RhB) and Sulforhodamine-101 (SR101)) can be regarded as a combination of a set of 2D luminous sections. Focusing on each 2D section and taking photos synchronously using two CCD cameras layer by layer, the original luminosity distribution of each section can be retrieved using the image inversion algorithm. Temperature-sensitive fluorescent dye (RhB) and temperature-insensitive fluorescent dye (SR101) are selected in tend to eliminate the effect caused by fluctuation of illuminating intensity. A temperature calibration method is put forward, and the influence of calibration results caused by uneven distribution of 3D temperature field in micro-channel is reduced. The calibration relationship between the ratio of intensities in different spectral bands and temperature is acquired, and the reconstruction of 3D temperature field is realized.