三邻甲苯磷酸酯诱导母鸡神经组织中自噬相关蛋白含量的变化

Objective To study the changes of autophagy-related proteins in the delayed neuropathy (OPIDN) induced by hree-o-cresyl phosphate (TOCP), and explore the molecular mechanism of OPIDN. Methods Thirty adult Roman hens were randomly divided into groups of control, 1d, 5d, 10d and 21d（6 hens per group）. Except the group control，the rest were intoxicated with TOCP by gavage at a single dosage of 750 mg/kg. The level of Atg1, Atg5 and Beclin1 in tibial nerves and spinal cords was determined by immunoblotting. Results In tibial nerves, compared to the control group, the level of Atg1 decreased by 29.8%, 64.4%, 43.5% and 19.8% respectively on 1d, 5d, 10d and 21d（P<0.05）; the level of Atg5 decreased by 36.8%, 49.6%, 51.2% and 31.5% respectively on 1d, 5d, 10d and 21d（P<0.05）; the level of Beclin1 decreased by 68.5%, 66.3% and 32.2 respectively on 1d, 5d and 10d（P<0.05）. In spinal cords , compared to the control group, the level of Atg1 decreased by 23.5%, 48.7% and 20% respectively on 1d, 5d and 10d（P<0.05）; the level of Atg5 decreased by 32.7%, 51.5%,47.3% and 39.6% respectively on 1d , 5d, 10d and 21d（P<0.05）; the level of Beclin1 decreased by 28.9%, 50.2%, 43.2% and 28.3% respectively on 1d, d5, 10d and 21d（P<0.05）. Conclusion The intoxication of TOCP was associated with the significant change of autophagy-related proteins in hen nervous tissues, which might be involved in the pathogenesis of OPIDN.