双功能抗体募集分子DNP-LHRH的合成

Luteinizing hormone releasing hormone (LHRH) is a polypeptide consisting of 10 amino acids that specifically binds onto cancer cells overexpressing LHRH receptors, and then it has been used in the development of targeted anti-tumor drugs. In this paper, the LHRH peptide analogue with azide group was synthesized by peptide solid phase synthesis method. Secondly, 2,4-dinitrobenzene (DNP) derivativescontaining 2, 4 and 8 ethylene glycol repeat unit linkerwere synthesized respectively.Finally, DNP derivatives were coupled to the LHRH analogto acquire antibody recruitment molecules DNP-LHRH. via a click chemical reaction. These bifunctional antibody recruiting moleculesare expected to combine with anti-DNP antibodies in serum and then deliver to overexpressing LHRH receptors cancer cells surface, which would be destroyedby exciting the immune response.