不同Wnt信号转导通路抑制剂对HL60细胞增殖和凋亡的影响

Objective: To investigate the effects of different Wnt signaling pathway inhibitors on the proliferation and apoptosis of HL60 cells. To explore the role of Wnt signaling pathway in acute leukemia cells proliferation. Methods: HL60 cells were incubated with different Wnt signaling pathway inhibitors (XAV939, ICG001). Cell proliferation was examined by using CCK8. Cell apoptosis was analyzed by FACS byusing PE-ANNEXIN V/7-AAD antibody. Results: 1、After HL60 cells were incubated with a beta-catenin inhibitor, ICG001, at different concentrations (0uM、10uM、50uM) for 24 or 48 hours, the proliferation of HL60 cells decreased significantly with the increasing concentration of ICG001 (P<0.05). When HL60 cells were incubated with ICG001 at the same concentration, the cell proliferation index was significantly decreased after 48 hours comparing to that after 24 hours (P<0.05), indicating that ICG001 inhibited HL60 cells proliferation in a time-dependent manner. ICG001 could also induce HL60 cells apoptosis. The cells apoptosis increased significantly with the increasing of ICG concentration and incubation time. 2、XAV939 （a regulator of Axin）didn't interfere with the proliferation of HL60 cells. Even though the XAV939 concentration and incubation time were increased, or FBS concentration was decreased in the cell medium, HL60 cells proliferation was not affected by XAV939. As well, XAV939 had no effects on HL60 cells apoptosis. Conclusion: Wnt signaling pathway regulates the proliferation and apoptosis of acute leukemia cells. Meanwhile, there may be some mutant proteins involved in Wnt signaling pathway in HL60 cells, leading to the different effects of ICG001 and XAV939 on cell proliferation and apoptosis.