酿酒葡萄原花色素生物合成关键结构基因VvLAR和VvANR启动子的克隆与功能预测
Cloning and Functional Prediction for Promoters of VvLAR and VvANR Which Are Key Structure Genes Related to Proanthocyanidin Biosynthesis in Wine Grapes
原花色素又称缩合单宁，为黄烷醇缩合而成的寡聚体或多聚体，是葡萄果实中的一类重要的多酚次生代谢产物。原花色素不仅具有很强生理活性和对人体多种潜在的保健作用，而且对葡萄和葡萄酒的口味有着重要的影响。近年来，一系列涉及葡萄果实原花色素生物合成的关键酶及其生理功能已经被逐渐阐明，而到目前为止对这些基因启动子结构和功能的研究还非常有限。本研究旨在通过生物信息学的方法预测原花色素生物合成关键酶无色花色素还原酶（Leucoanthocyanidin reductase; LAR）和花色素还原酶（Anthocyandin reductase, ANR）基因的启动子序列，设计相应的特异引物并将其克隆，通过生物信息学方法对它们启动子的确切序列进行比对和分析，对其潜在功能进行预测。本研究发现，与原花色素生物合成有关的这些关键结构基因的启动子中，富含大量潜在的顺式作用元件或顺式作用元件的部分配件，其中多种与光响应有关，表明这些关键基因的表达可能极大的受到光信号诱导的影响。
分子生物学生物化学植物学
葡萄原花色素无色花色素还原酶花色素还原酶启动子克隆
李强,何非,潘秋红,段长青,朱保庆.酿酒葡萄原花色素生物合成关键结构基因VvLAR和VvANR启动子的克隆与功能预测[EB/OL].(2013-03-05)[2025-10-10].http://www.paper.edu.cn/releasepaper/content/201303-124.点此复制
Proanthocyanidins, also known as condensed tannins, are oligomers or polymers of flavanols, which are a group of important secondary polyphenolic metabolites in grape berries. Proanthocyanidins do not only have strong physiology activities and potential health effects to human body, but also have great influence on the taste of grape and wine. In recent years, a series of key enzymes related to proanthocyanidin biosynthesis and their physical functions were gradually clarified, but until now the researches for the structures and functions of the promoters of these genes are still quite limited. In the present research, the promoter sequences of the genes of Leucoanthocyanidin Reductase (LAR) and Anthocyandin Reductase (ANR) related to proanthocyanidin biosynthesis were predicted with the help of bioinformatics, which were cloned by using specifically designed primers. Their promoters' exact sequences were compared and analyzed with the help of bioinformatics, and their potential functions were predicted. This research revealed that there were a large number of potential cis-acting elements or their subunits in the promoters of these key structural genes related to proanthocyanidin biosynthesis, lots of which were related to photoresponse, showing that the expression of these genes might be affected by the photosignal introduction.
GrapeProanthocyanidinLeucoanthocyanidin ReductaseAnthocyanidin ReductasePromoterCloning
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