电针对SAMP8小鼠海马区PS1蛋白表达的影响

IABSTRACT Objective: To research the efffect of Electro-acupuncture on the PS1 protein expression in the hippocampus of mice SAMP8, and discuss the mechanism of the EA treatment on AD. Methods: Twenty 8-month-old SAMP8 mice were randomly divided into model group and EA group, with ten SAMR1 mice as the normal control group. All the groups feed in normal environment. Model group and normal group should be caught and bounded once when the EA group is being treated. "Baihui"、"Yintang"、"Renzhong" were selected as the EA treat points for EA group 20 min a day for 15 days. The current frequency is 2 Hz and current strength is appropriate when the head of mice is quivering. The learning-memorize ability of mice was assessed by Morris water maze when the treatment finished. Immunohistochemistral method was used to observe the PS1 protein expression in the hippocampus of mice. The contents of PS1 protein in hippocampus was tested by Western Blotting. Results：The Morris water maze test showed the escape latency of model group increased ,the number of platform-site crossover and the swimming duration in platform quadrant of model group were reduced compared with the control group（P<0.05， P<0.01）; the escape latency of EA group reduced, while the crossing time and dwell time in the target quadrant increased compared with the model group(P<0.05). The Immunohistochemistral result showed that the PS1 protein in the hippocampus of EA group obviously decreased compared with the model group（P<0.01）. The Western Blotting result showed the contents of PS1 protein in the model group were higher than that in the control group（P<0.01）, while the EA group could reduce its expression（P<0.01). Conclusions: EA therapy can improve the learning-memorize ability of the SAMP8 mice and decrease the protein contents of Aβand PS1 in hippocampus. The EA treatment might be a promising method to AD patients.