Temperature increase affects acetate-derived methane production in Alaskan lake sediments and wetland soils
Temperature increase affects acetate-derived methane production in Alaskan lake sediments and wetland soils
Abstract Under climate change framework, methanogens activity is expected to be strongly affected, eventually resulting in positive feedback on global climate, with higher greenhouse gas (GHG) emissions in the Arctic. This work aimed to evaluate the effect of increasing temperature on methane production rate and archaeal community of lake sediments and wetland soils from Denali to Toolik regions in Alaska (USA). For that, anaerobic acetate-amended microcosms were incubated at 5, 10, 15 and 20 °C. The acetate-derived methanogenic rate was determined and the methanogenic communities were analyzed by qPCR and 16S rRNA sequencing. Warmer temperatures yielded 4-6 times higher methane production rates and organic matter content (OM) showed significant positive correlation to methane production. Different patterns were observed in the archaeal communities after incubation at higher temperatures, with an increase in Methanosarcina abundance for most of the samples and Methanosaeta in one of the lakes tested, showing the adaptation of key acetoclastic groups among different temperatures. Our results demonstrate the impact of increasing temperature on methane production, bringing insights on key drivers involved in the process of acetoclastic methanogenic potential occurring in these ecosystems in Alaska.
Dellagnezze BM、Lavergne C.、Mosquillo F.、Etchebehere C.、Cabrol L、Barret M.、Bovio-Winkler P.、Menoni D.A.
Microbial Ecology Laboratory, Department of Microbial Biochemistry and Genomic, Biological Research Institute ?°Clemente Estable,?± MontevideoHUB AMBIENTAL UPLA, Laboratory of Aquatic Environmental Research, Centro de Estudios Avanzados, Universidad de Playa Ancha||Escuela Ingenier¨aa Bioqu¨amica, Pontificia Universidad Cat¨?lica de Valpara¨asoMicrobial Ecology Laboratory, Department of Microbial Biochemistry and Genomic, Biological Research Institute ?°Clemente Estable,?± MontevideoMicrobial Ecology Laboratory, Department of Microbial Biochemistry and Genomic, Biological Research Institute ?°Clemente Estable,?± MontevideoEscuela Ingenier¨aa Bioqu¨amica, Pontificia Universidad Cat¨?lica de Valpara¨aso||Aix-Marseille Universit¨|, Univ Toulon, CNRS, IRD ¨C Mediterranean Institute of Oceanography||Institute of Ecology and Biodiversity IEB, Faculty of Sciences, Universidad de ChileLaboratoire ??Ecologie Fonctionnelle et Environnement, Universit¨| de ToulouseMicrobial Ecology Laboratory, Department of Microbial Biochemistry and Genomic, Biological Research Institute ?°Clemente Estable,?± MontevideoMicrobial Ecology Laboratory, Department of Microbial Biochemistry and Genomic, Biological Research Institute ?°Clemente Estable,?± Montevideo
环境科学理论环境科学技术现状环境生物学
methanefreshwater ecosystemsclimate change16S rRNA amplicons
Dellagnezze BM,Lavergne C.,Mosquillo F.,Etchebehere C.,Cabrol L,Barret M.,Bovio-Winkler P.,Menoni D.A..Temperature increase affects acetate-derived methane production in Alaskan lake sediments and wetland soils[EB/OL].(2025-03-28)[2025-05-06].https://www.biorxiv.org/content/10.1101/2021.08.22.457279.点此复制
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