高产精氨酸脱亚胺酶菌株的复合诱变选育及其发酵工艺优化

o improve the arginine deiminase producing ability, Enterococcus faecalis SK 32.001 was mutated using chemical method coupled with atmospheric room temperature plasmas. A high arginine deiminase producing strain A3 was obtained by plate initial screening and re-screening in shake flask fermentation. Then the enzyme producing ability of the strains was further improved by optimizing fermentation conditions. The results showed that the enzyme activity of the mutant strain A3 was 3.12 U/mL with sucrose and beef extract as carbon and nitrogen sources, respectively, and with the dissolved oxygen value was controlled at 20%. Compared with the original strain, the enzyme activity was improved by 162.18%.