Opto-lipidomics of tissues
Opto-lipidomics of tissues
Abstract Lipid metabolism and signalling play pivotal functions in biology and disease development. Despite this, there is currently no optical technique available that can directly visualise the lipidome in tissues. In this study, we introduce opto-lipidomics, a new approach to optical molecular tissue imaging. We expand the capability of vibrational Raman spectroscopy to identify individual lipids in complex tissue matrices through correlation with desorption electrospray ionisation (DESI) - mass spectrometry imaging in an integrated instrument. A computational pipeline of inter-modality regression analysis is established to extract lipidomic information from optical vibrational spectra. Opto-lipidomic imaging of transient cerebral ischemia-reperfusion injury in a murine model of ischemic stroke demonstrates the visualisation and identification of lipids in disease with unprecedented molecular specificity using light. Furthermore, we deploy opto-lipidomics in a handheld fiber-optic Raman probe and demonstrate real-time classification of bulk brain tissues based on specific lipid abundances. Opto-lipidomics opens a host of opportunities to study lipid biomarkers for diagnostics, prognostics, and novel therapeutic targets.
Birjandi Anahid A.、Jensen Magnus、Stepula Elzbieta、Chan Ka Lung Andrew、Parsons Maddy、Chapple Sarah J.、Martella Davide、Abbate Vincenzo、Farrell-Dillon Keith、Vercauteren Tom、Bergholt Mads S.、Chiappini Ciro、Mann Giovanni E.、Liu Shiyue
Centre for Craniofacial and Regenerative Biology, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College LondonInstitute of Pharmaceutical Science, King?ˉs College LondonRandall Centre for Cell and Molecular Biophysics, King?ˉs College LondonKing?ˉs British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College LondonDepartment of Analytical, Environmental and Forensic Sciences, King?ˉs College LondonKing?ˉs British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King?ˉs College LondonSchool of Biomedical Engineering and Imaging Sciences, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College LondonKing?ˉs British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King?ˉs College LondonCentre for Craniofacial and Regenerative Biology, King?ˉs College London||Institute of Pharmaceutical Science, King?ˉs College London
基础医学生物科学研究方法、生物科学研究技术生物化学
Opto-lipidomicsRaman spectroscopymass spectrometry
Birjandi Anahid A.,Jensen Magnus,Stepula Elzbieta,Chan Ka Lung Andrew,Parsons Maddy,Chapple Sarah J.,Martella Davide,Abbate Vincenzo,Farrell-Dillon Keith,Vercauteren Tom,Bergholt Mads S.,Chiappini Ciro,Mann Giovanni E.,Liu Shiyue.Opto-lipidomics of tissues[EB/OL].(2025-03-28)[2025-05-16].https://www.biorxiv.org/content/10.1101/2023.04.01.534702.点此复制
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