一株藻际细菌Cyclobacterium xiamenense 的分离鉴定

In an attempt to investigate the relationship between bacteria and alga, many bacteria strains from the phycospher were isolated and characterized. This study involved one strain, designated strain KD51 which was isolated from the phycospher of alga Chlorella autotrophic. 16S rRNA gene sequence comparison showed that strain KD51T is a member of the genus Cyclobacterium, forming a distinct lineage with Cyclobacterium lianum HY9T. The 16S rRNA gene sequence similarity between strain KD51T and the type strains of Cyclobacterium species ranged from 93.6 % to 95.2%. The DNA G+C content was 41.7 mol% and the major respiratory quinone was menaquinone-7 (MK-7). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphotidyl inositol, aminolipids, unidentified polar lipid and unidentified phospholipioides. On the basis of phenotypic data and phylogenetic inference, strain KD51T represents a novel species, for which the name Cyclobacterium xiamenense sp.nov. is proposed.