miRNA-126-3p与野百合碱诱导大鼠肺动脉高压发病机制研究

Background: Our previous studies have found that the miRNA-126-3p may participate in pulmonary hypertension (PH) of congenital heart disease by regulating VEGFA; this study intends to validate it by animal models. Objective: To explore whether miRNA-126-3p is related to the occurrence of PH in rats. Methods: The experimental group was (15 male SD rats) induced by monocrotaline, and control group had 10 rats. Four weeks later, the qRT-PCR detection of the lung tissue expression of miRNA-126-3p was done, and the level of mRNA and protein level detection of VEGFA also was tested. Results: There were 8 rats which were successfully induced by monocrotaline, and the average pulmonary artery pressure had obvious difference than the control group (p<0.0001); miRNA-126-3p expression level was significantly statistical difference (p<0.0001) between the two groups; VEGFA expression at mRNA level and protein level were statistically significantly than control group (p<0.0001). Conclusion: miRNA-126-3p may participate in monocrotaline induced PH in rats by regulating VEGFA.