新型Cys荧光探针的设计、合成与应用研究

In this work, a novel two-photon probe P2 has been developed for cysteine (Cys) detection. The probe was based on introducing a Cys-triggered acryloyl group to the two-photon fluorophore 1-(6-hydroxynaphthalen-2-yl) ethanone. The probe P2 itself exhibited weak fluorescence, however, it displayed a dramatic fluorescence enhancement at 455 nm responding towards Cys (about 20 fold) and the high signal to background ratio enables a low limit of detection(S/N=3) to be 0.10 μmol/L. In adittion, the probe presents a fast response which the fluorescence intensity of the probe tends to stable in 28 minutes, high stability and high selectivity towards Cys at physiological pH conditions.More meaningfully, the probe P2 could be used for two-photon imaging of Cys in living cells with high selectivity, low toxicity and good biocompatibility.