5-羟甲基胞嘧啶上调参与抑郁样行为的发生

Objective: To observe the distribution of 5-hmC in mice brain and the change of 5-hmC in the depressive mice, then to explore the possible pathogenesis of depressive phenotype. Methods: Heart perfusion and frozen section technique were used to normal adult male ICR mice;Then we used immunohistochemical staining method to obeserve the distribution of 5-hmC in brain tissue. Immunofluorescent staining and Dot blot were used to obeserve the distribution of 5-hmC in mitochondria. Chronic restraint stress was used to establish mouse model of depression. Tail suspension test and forced swimming test were used to examine depressive phenotype in mice. Using Dot blot and IHC techniques to detect the contents of 5-hmC in the brain tissue.Results: (1) DAB staining of normal male ICR mice intuitively showed the distribution of 5-hmC in the brain tissue; 5-hmC expressed rich in the cerebral cortex , hippocampus , hypothalamus , amygdala , striatum , cerebellum and brain stem; In addition, it has expressed in nuclear genes and mitochondrial DNA. (2) Tail suspension test and forced swimming test showed that the immobility time of Stress group were significantly increased compared with the Control group (P<0.05).(3) Dot blot showed that the level of 5-hmC in brain tissue of stress group increased significantly (P<0.05). After the depression , Immunohistochemical staining showed that 5-hmC increased in the cerebral cortex , hypothalamus , hippocampus and amygdala(P<0.05).