胶原蛋白肽对蛋氨酸限制高脂饮食小鼠骨骼的影响

Objective: To investigate the effects of collagen peptide on body weight, bone strength, bone tissue redox state and microstructure in mice fed with methionine restricted and high fat diet. Methods: 36 male C57BL/6 mice were randomly divided into methionine restricted diet(MR) and collagen peptide-methionine restricted diet(PMR). In the 22th week, mice energy metabolism was measured by comprehensive laboratory animal monitoring system(CLAMS) and then the mice sacrificed. Remove the femur, scanning femur microstructure, the length of femur, bone dry weight, bone mineral content, bone mineral density, bone strength, bone tissue redox state, the expression of osteoblast and osteoclast differentiation related genes were determined. Results: Compared with MR group, the body weight, energy expenditure, bone dry weight, bone mineral content, bone volume, bone mineral density, trabecular number, bone strength, bone strength/body weight significantly decreased（P<0.05）. Trabecular separation and the osteoclast differentiation related genes significantly increased（P<0.05）. Conclusion: The addition of dietary collagen peptide can reduce energy expenditure and bone mineral content, damage the microstructure of bone tissue, reduce bone mineral density, thereby reduce bone strength and destroy the balance between osteoblasts and osteoclasts in mice fed methionine restricted-high fat diet.Therefore, at the dietary methionine restriction, the addition of collagen peptides may have adverse effects on the mice bone.