辛伐他汀干预骨质疏松大鼠骨髓基质细胞后microRNA的差异表达研究

Objective:This study aims to study the potential mechanisms that how Simvastatin promotes stromal cell in bone marrow to bone and how osteoporosis occurs, according to investigating miRNAs expression. Method: When the whole bone marrow culture method in vitro culture of bone marrow stromal cells, bone marrow stromal cell expansion to the third generation, were divided into three treatment groups: Osteoporosis bone marrow stromal cells plus simvastatin group (OP + SIM), osteoporosis bone marrow stromal cells group (OP + K), normal bone marrow stromal cells group (N + K).To culture day 10 rows miRNA microarray RNA extracted each sample to detect miRNA expression levels.Result: MiRNA microarray assay showed that 20 microRNAs' expression was up-regulated and 27 microRNAs was down-regulated in OP+SIM VS OP+K. In addition, there were 27 microRNAs in up-regulated expression and 27 microRNAs in down-regulated expression in OP+K VS N+K.Conclusion: Simvastatin may direct intervention by the expression levels of miRNA adjusted osteogenic potential of bone marrow stromal cells. Osteoporosis source of bone marrow stromal cells as compared with normal bone marrow stromal cells with a series of miRNA expression levels change