彗星电泳法测定双酚A对小鼠脑细胞的DNA损伤

Objective: This study was aimed to investigate the DNA damages of brain cells in mice induced by long-term exposure to Bisphenol A. Methods: Four mice groups, including the control group and three exposure groups, chronically received different Bisphenol A treatments (0μg/mL, 0.1μg/mL, 10μg/mL and 1000μg/mL in tea oil) at Am 9:00-11:00 for 8 weeks. Then comet assay was employed to detect the DNA damages of the brain cells in these mice. Results: After the treatment with Bisphenol A, the comet percentages of brain cells increased from 9.5% in the control group to 34.5%, 36.0% and 50.5% in three exposure groups, respectively. And the percentages of tail DNA, tail length and tail moment in brain cells in the exposure groups were significantly higher than those in the control group (p< 0.01). Conclusion: The result showed that long-term exposure to Bisphenol A, including the low level exposure, could lead to DNA damages of the brain cells in mice and the damage levels increased with the increasing of Bisphenol A concentrations.