Inducing Human Retinal Pigment Epithelium-like Cells from Somatic Tissue
Tachibana Akihiro 1Sahara Yoshiki 2Kaczkowski Bogumil 3Sakai Noriko 1Hu Haiming 1Takasato Minoru 4Maeda Akiko 1Arner Erik 3Nishida Mitsuhiro 1Kasukawa Takeya 3Sho Junki 1Takahashi Masayo 1Kime Cody 1Abugessaisa Imad 3Carninci Piero 3Hasegawa Akira 3Mandai Michiko 1Hon Chung-Chau 3Woogeng Ivo Ngundu 1Sanyal Hashimita1
作者信息
- 1. RIKEN Center for Biosystems Dynamics Research
- 2. RIKEN Center for Biosystems Dynamics Research||Laboratory of Molecular Cell Biology and Development, Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University||Department of Renal and Cardiovascular Research, New Drug Research Division, Otsuka Pharmaceutical Co. Ltd.
- 3. RIKEN Center for Integrative Medical Sciences
- 4. RIKEN Center for Biosystems Dynamics Research||Laboratory of Molecular Cell Biology and Development, Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University
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Abstract
SUMMARY
Regenerative medicine relies on basic research to find safe and useful outcomes that are only practical when cost-effective. The human eyeball requires the retinal pigment epithelium (RPE) for support and maintenance that interfaces the neural retina and the choroid at large. Nearly 200 million people suffer from age-related macular degeneration (AMD), a blinding multifactor genetic disease among other retinal pathologies related to RPE degradation. Recently, autologous pluripotent stem cell-derived RPE cells were prohibitively expensive due to time, therefore we developed a new simplified cell reprogramming system. We stably induced RPE-like cells (iRPE) from human fibroblasts by conditional overexpression of broad plasticity and lineage-specific pioneering transcription factors. iRPE cells showed features of modern RPE benchmarks and significant in-vivo integration in transplanted chimeric hosts. Herein, we detail the iRPE system with comprehensive modern single-cell RNA (scRNA) sequencing profiling to interpret and characterize its best cells. We anticipate that our system may enable robust retinal cell induction for regenerative medicine research and affordable autologous human RPE tissue for cell therapy.Key words
cell biology/cell plasticity/reprogramming/regenerative medicine/retina引用本文复制引用
Tachibana Akihiro,Sahara Yoshiki,Kaczkowski Bogumil,Sakai Noriko,Hu Haiming,Takasato Minoru,Maeda Akiko,Arner Erik,Nishida Mitsuhiro,Kasukawa Takeya,Sho Junki,Takahashi Masayo,Kime Cody,Abugessaisa Imad,Carninci Piero,Hasegawa Akira,Mandai Michiko,Hon Chung-Chau,Woogeng Ivo Ngundu,Sanyal Hashimita.Inducing Human Retinal Pigment Epithelium-like Cells from Somatic Tissue[EB/OL].(2025-03-28)[2026-05-08].https://www.biorxiv.org/content/10.1101/2020.07.27.215103.学科分类
眼科学/基础医学/生物科学研究方法、生物科学研究技术
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